Uji Aktivitas Antibakteri Fraksi N-Heksan Ekstrak Etanol Biji Sirsak (Annona muricata Linn) Asal Tidore Terhadap Pseudomonas aeruginosa dengan metode Bioautografi

Test studies have been conducted Antibacterial Activity of n-Hexane fraction seed extract soursop (Annona muricata Linn) from Tidore against Pseudomonas aeruginosa with methods Bioautografi. The purpose of this study was to determine the compound contained in the ethanol extract Seeds Soursop (Annona muricata Linn ) from Tidore which can inhibit the growth of Pseudomonas aeruginosa with methods bioautgrafi. Results TLC (thin layer chromatography) obtained from n- hexan fraction of ether extract Seeds Soursop (Annona muricata Linn) showed seven staining with Rf1 value of 0.98 (orange colored stains ) ; Rf2 0.82 ( light green stain ) and Rf3 0.78 (dark green stain) . ; Rf4 0.67 (light green stain) ; Rf5 0.69 (dark green stain) ; Rf6 0.57 (light green stain) and Rf7 0.30 (yellow stain) on stain UV 366 nm. Based on the results Bioautografi shows one bioactive compounds from the extracts of ether seeds soursop (Annona muricata Linn) from Tidore can inhibit the growth of Pseudomonas aeruginosa spotting Rf4 value of 0.67 (light green stain) with a diameter of 8.14 mm inhibition zone Keywords: Antibacterial, Seeds Soursop (Annona muricata Linn), Pseudomonas aeruginosa, Bioautografi